富山県立大学 工学部生物工学科・生物工学専攻 機能性食品工学

主な発表論文

2024

Mukai R, Okuyama H, Uchimura M, Sakao K, Matsuhiro M, Ikeda-Imafuku M, Ishima Y, Nishikawa M, Ikushiro S, Tai A. The binding selectivity of quercetin and its structure-related polyphenols to human serum albumin using a fluorescent dye cocktail for multiplex drug-site mapping. Bioorg Chem. 145:107184. (2024)

Mitsuzane R, Okubo R, Nishikawa M, Ikushiro S, Munemasa S, Murata Y, Nakamura Y, Nakamura T. Enhancing effect of the coexisting alpha-tocopherol on quercetin absorption and metabolism. Free Radic Res. 8:1-16. (2024) 

Ikushiro H, Honda T, Murai Y, Murakami T, Takahashi A, Sawai T, Goto H, Ikushiro SI, Miyahara I, Hirabayashi Y, Kamiya N, Monde K, Yano T. Racemization of the substrate and product by serine palmitoyltransferase from Sphingobacterium multivorum yields two enantiomers of the product from D-serine. J Biol Chem. 5:105728. (2024)

2023

Iwai Y, Iijima A, Kise S, Nagao C, Senda Y, Yabu K, Mano H, Nishikawa M, Ikushiro S, Yasuda K, Sakaki T.Characterization of Rickets Type II Model Rats to Reveal Functions of Vitamin D and Vitamin D Receptor. Biomolecules. 13:1666. (2023)

Kise S, Iijima A, Nagao C, Okada T, Nishikawa M, Ikushiro S, Nakanishi T, Sato S, Yasuda K, Sakaki T. Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector. Sci Rep. 13:18528. (2023) 

Ishibashi R, Matsuhisa R, Nomoto M, Chudan S, Nishikawa M, Tabuchi Y, Ikushiro S, Nagai Y, Furusawa Y. Effect of Oral Administration of Polyethylene Glycol 400 on Gut Microbiota Composition and Diet-Induced Obesity in Mice. Microorganisms. 11:1882. (2023)

Kushioka T, Mano H, Matsuoka S, Nishikawa M, Yasuda K, Ikushiro S, Sakaki T. Analysis of vitamin D metabolites in biological samples using a nanoluc-based vitamin D receptor ligand sensing system: NLucVDR. J Steroid Biochem Mol Biol. 233:106367. (2023)

Kato Y, Sakanishi A, Matsuda K, Hattori M, Kaneko I, Nishikawa M, Ikushiro S. Covalent adduction of serotonin-derived quinones to the SARS-CoV-2 main protease expressed in a cultured cell. Free Radic Biol Med. 206:74-82. (2023)

Suzuki A, Kamakura M, Shiramata T, Nakaoka S, Sakamoto Y. Comparison of RNA-Seq analysis data between tracheal mite-infested and uninfested Japanese honey bees (Apis cerana japonica). BMC Res Notes. 6(1):122. (2023)

Lyu J, Kubo T, Iwahashi S, Fukasawa K, Horie T, Nagamatsu K, Ikeno K, Nakamura G, Kamakura M, Hinoi E.Amelioration of Osteoarthritis Development by Daily Oral Supplementation of Royal Jelly. Biol Pharm Bull. 246:348-353. (2023)

Nagao C, Kise S, Iijima A, Okada T, Nakanishi T, Sato S, Nishikawa M, Ikushiro S, Yasuda K, Sakaki T. Expression of Rat Cyp27b1 in HepG2 Cells Using Adenovirus Vector and Its Application to Evaluation of Self-Made and Commercially Available Anti-Cyp27b1 Antibodies. J Nutr Sci Vitaminol. 69:90-97. (2023)

Chudan S, Ishibashi R, Nishikawa M, Tabuchi Y, Nagai Y, Ikushiro S, Furusawa Y. Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells. Molecules. 28:3079. (2023)

Dong W, Akasaka I, Komiyama A, Nakamura T, Mizoguchi N, Nawaji T, Ikushiro S, Kobayashi M, Teraoka H. Augmentation of Pectoral Fin Teratogenicity by Thalidomide in Human Cytochrome P450 3A-Expressing Zebrafish. Pharmaceuticals. 16:368. (2023)

Kise S, Iijima A, Nagao C, Okada T, Mano H, Nishikawa M, Ikushiro S, Kanemoto Y, Kato S, Nakanishi T, Sato S, Yasuda K, Sakaki T. Functional analysis of vitamin D receptor (VDR) using adenovirus vector. J Steroid Biochem Mol Biol. 230:106275. (2023)

Sato Y, Dong W, Nakamura T, Mizoguchi N, Nawaji T, Nishikawa M, Onaga T, Ikushiro S, Kobayashi M, Teraoka H. Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina. Int J Mol Sci. 24:4013. (2023)

Chudan S, Ishibashi R, Nishikawa M, Tabuchi Y, Nagai Y, Ikushiro S, Furusawa Y. Effect of soluble oat fiber on intestinal microenvironment and TNBS-induced colitis. Food Funct. 14:2188-2199. (2023)

Fujiwara N, Mukai R, Nishikawa M, Ikushiro S, Murakami A, Ishisaka A. Transfer of quercetin ingested by maternal mice to neonatal mice via breast milk. Biosci Biotechnol Biochem. 87:442-447. (2023)

Mano H, Kushioka T, Kise S, Nagao C, Iijima A, Nishikawa M, Ikushiro S, Yasuda K, Matsuoka S, Sakaki T. Development of nanoluciferase-based sensing system that can specifically detect 1α,25-dihydroxyvitamin D in living cells. J Steroid Biochem Mol Biol. 227:106233. (2023)

Tagawa K, Maruo Y, Mimura Y, Ikushiro S. Toxicol Mech Methods. Effects of common genetic variants of human uridine diphosphate glucuronosyltransferase subfamilies on irinotecan glucuronidation. 33:197-205. (2023)

2022

Yamanouchi Y, Chudan S, Ishibashi R, Ohue-Kitano R, Nishikawa M, Tabuchi Y, Kimura I, Nagai Y, Ikushiro S, Furusawa Y. The Impact of Low-Viscosity Soluble Dietary Fibers on Intestinal Microenvironment and Experimental Colitis: A Possible Preventive Application of Alpha-Cyclodextrin in Intestinal Inflammation. Mol Nutr Food Res. 66:e2200063. (2022)

Nishikawa M, Kada Y, Kimata M, Sakaki T, Ikushiro S. Comparison of metabolism and biological properties among positional isomers of quercetin glucuronide in LPS- and RANKL-challenged RAW264.7 cells. Biosci Biotechnol Biochem.86:1670-1679. (2022) 

Hanioka N, Tanaka-Kagawa T, Mori Y, Ikushiro S, Jinno H, Ohkawara S, Isobe T. Regioselective Glucuronidation of Flavones at C5, C7, and C4' Positions in Human Liver and Intestinal Microsomes: Comparison among Apigenin, Acacetin, and Genkwanin. Biol Pharm Bull. 45:1116-1123. (2022)

Nishikawa M, Murose N, Mano H, Yasuda K, Isogai Y, Kittaka A, Takano M, Ikushiro S, Sakaki T. Robust osteogenic efficacy of 2α-heteroarylalkyl vitamin D analogue AH-1 in VDR (R270L) hereditary vitamin D-dependent rickets model rats. Sci Rep. 12:12517. (2022)

Yogo Y, Yasuda K, Takita T, Yasukawa K, Iwai Y, Nishikawa M, Sugimoto H, Ikushiro S, Sakaki T. Metabolism of non-steroidal anti-inflammatory drugs (NSAIDs) by Streptomyces griseolus CYP105A1 and its variants. Drug Metab Pharmacokinet. 45:100455. (2022)

Tanaka Y, Okuyama H, Nishikawa M, Ikushiro SI, Ikeda M, Ishima Y, Ukawa Y, Oe K, Terao J, Mukai R. 8-Prenylnaringenin tissue distribution and pharmacokinetics in mice and its binding to human serum albumin and cellular uptake in human embryonic kidney cells. Food Sci Nutr. 10:1070-1080. (2022) 

Ishibashi R, Furusawa Y, Honda H, Watanabe Y, Fujisaka S, Nishikawa M, Ikushiro S, Kurihara S, Tabuchi Y, Tobe K, Takatsu K, Nagai Y. Isoliquiritigenin Attenuates Adipose Tissue Inflammation and Metabolic Syndrome by Modifying Gut Bacteria Composition in Mice. Mol Nutr Food Res. 66:e2101119. (2022)

Takita T, Sakuma H, Ohashi R, Nilouyal S, Nemoto S, Wada M, Yogo Y, Yasuda K, Ikushiro S, Sakaki T, Yasukawa K. Comparison of the stability of CYP105A1 and its variants engineered for production of active forms of vitamin D. Biosci Biotechnol Biochem. 86:444-454. (2022)

Tanaka S, Trakooncharoenvit A, Nishikawa M, Ikushiro S, Hara H. Heteroconjugates of quercetin with 4'-O-sulfate selectively accumulate in rat plasma due to limited urinary excretion. Food Funct. 13:1459-1471. (2022)

2021

Kato Y, Higashiyama A, Takaoka E, Nishikawa M, Ikushiro S. Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro. Adv Redox Res. 3:100021. (2021)

Yasuda K, Nishikawa M, Mano H, Takano M, Kittaka A, Ikushiro S, Sakaki T. Development of In Vitro and In Vivo Evaluation Systems for Vitamin D Derivatives and Their Application to Drug Discovery. Int J Mol Sci. 22:11839. (2021)

Yasuda K, Watanabe K, Fukami T, Nakashima S, Ikushiro SI, Nakajima M, Sakaki T. Epicatechin gallate and epigallocatechin gallate are potent inhibitors of human arylacetamide deacetylase. Drug Metab Pharmacokinet. 39:100397. (2021)

Yasuda K, Nishikawa M, Okamoto K, Horibe K, Mano H, Yamaguchi M, Okon R, Nakagawa K, Tsugawa N, Okano T, Kawagoe F, Kittaka A, Ikushiro S, Sakaki T. Elucidation of metabolic pathways of 25-hydroxyvitamin D3 mediated by CYP24A1 and CYP3A using Cyp24a1 knockout rats generated by CRISPR/Cas9 system. J Biol Chem. 296:100668. (2021)

Yamaga M , Tani H , Nishikawa M , Fukaya K , Ikushiro SI , Murota.Pharmacokinetics and metabolism of cinnamic acid derivatives and flavonoids after oral administration of Brazilian green propolis in humans. Food Funct. 12:2520-2530. (2021) 

Uno Y, Mikami T, Tsukazaki Y, Nakanishi Y, Murayama N, Ikushiro S, Tsusaki H, Yamazaki H. Genetic variants of UDP-glucuronosyltransferases 1A1, 1A6, and 1A9 in cynomolgus and rhesus macaques. Xenobiotica. 51:115-121. (2021)

2020

Edamatsu H, Yagawa M, Ikushiro S, Sakaki T, Nakagawa Y, Miyagawa H, Akamatsu M. Identification and in silico prediction of metabolites of tebufenozide derivatives by major human cytochrome P450 isoforms. Bioorg Med Chem. 28:115429. (2020) 

Tsuchiya Y, Hayashi M, Nagamatsu K, Ono T, Kamakura M, Iwata T, Nakashima T. The key royal jelly component 10-hydroxy-2-decenoic acid protects against bone loss by inhibiting NF-κB signaling downstream of FFAR4. J Biol Chem. 95(34):12224-12232.(2020) 

Masuyama Y, Nishikawa M, Yasuda K, Sakaki T, Ikushiro S. Whole-cell dependent biosynthesis of N- and S-oxides using human flavin containing monooxygenases expressing budding yeast. Drug Metab Pharmacokinet. 35:274-280. (2020)

Nishikawa M, Yasuda K, Takamatsu M, Abe K, Okamoto K, Horibe K, Mano H, Nakagawa K, Tsugawa N, Hirota Y, Horie T, Hinoi E, Okano T, Ikushiro S, Sakaki T. Generation of novel genetically modified rats to reveal the molecular mechanisms of vitamin D actions. Sci Rep. 10:5677. (2020)

Uno Y, Uehara S, Inoue T, Kawamura S, Murayama N, Nishikawa M, Ikushiro S, Sasaki E, Yamazaki H. Molecular characterization of functional UDP-glucuronosyltransferases 1A and 2B in common marmosets. Biochem Pharmacol. 172:113748.(2020)

Miyauchi Y, Kurita A, Yamashita R, Takamatsu T, Ikushiro S, Mackenzie PI, Tanaka Y, Ishii Y. Hetero-oligomer formation of mouse UDP-glucuronosyltransferase (UGT) 2b1 and 1a1 results in the gain of glucuronidation activity towards morphine, an activity which is absent in homo-oligomers of either UGT. Biochem Biophys Res Commun. 525:348-353. (2020) 

2019

Kato Y, Kawai M, Kawai S, Okano Y, Rokkaku N, Ishisaka A, Murota K, Nakamura T, Nakamura Y, Ikushiro S. Dynamics of the Cellular Metabolism of Leptosperin Found in Manuka Honey. J Agric Food Chem. 67:10853-10862. (2019)

Han JP, Zhu ZH, Wu YZ, Qian W, Li ZY, Nishikawa M, Sakaki T, Yang CQ. Preparation of a Major Metabolite of Iguratimod and Simultaneous Assay of Iguratimod and Its Metabolite by HPLC in Rat Plasma. Iran J Pharm Res. 18:631-641. (2019)

Uno Y, Takahira R, Murayama N, Onozeki S, Kawamura S, Uehara S, Ikenaka Y, Ishizuka M, Ikushiro S, Yamazaki H. Functional and molecular characterization of UDP-glucuronosyltransferase 2 family in cynomolgus macaques. Biochem Pharmacol. 163:335-344. (2019)

Ono Y, Sugiyama S, Matsushita M, Kitazawa T, Amano T, Uno Y, Ikushiro S, Teraoka H. Limited expression of functional cytochrome p450 2c subtypes in the liver and small intestine of domestic cats. Xenobiotica. 49(6):627-635. (2019)

Tanaka S, Trakooncharoenvit A, Nishikawa M, Ikushiro S, Hara H.Comprehensive Analyses of Quercetin Conjugates by LC/MS/MS Revealed That Isorhamnetin-7- O-glucuronide-4'- O-sulfate Is a Major Metabolite in Plasma of Rats Fed with Quercetin Glucosides. J Agric Food Chem. 67:4240-4249. (2019)

Yasuda K, Okamoto K, Ueno S, Itoh K, Nishikawa M, Ikushiro S, Sakaki T. Sulfate conjugates are the major metabolites in rats administrated with sesamin. Drug Metab Pharmacokinet. 34:134-140. (2019)

Nishikawa M, Yasuda K, Takamatsu M, Abe K, Nakagawa K, Tsugawa N, Hirota Y, Tanaka K, Yamashita S, Ikushiro S, Suda T, Okano T, Sakaki T. Generation of 1,25-dihydroxyvitamin D3 in Cyp27b1 knockout mice by treatment with 25-hydroxyvitamin D3 rescued their rachitic phenotypes. J Steroid Biochem Mol Biol. 185:71-79. (2019)

2018

Mano H, Ikushiro S, Sakaki T. Novel split luciferase-based biosensors for evaluation of vitamin D receptor ligands and their application to estimate CYP27B1 activity in living cells. J Steroid Biochem Mol Biol. 183:221-227. (2018)
 

Uno Y, Takahira R, Murayama N, Ishii Y, Ikenaka Y, Ishizuka M, Yamazaki H, Ikushiro S. Molecular and functional characterization of UDP-glucuronosyltransferase 1A in cynomolgus macaques. Biochem Pharmacol. 155:172-181. (2018)

Matsumura E, Nakagawa A, Tomabechi Y, Ikushiro S, Sakaki T, Katayama T, Yamamoto K, Kumagai H, Sato F, Minami H. Microbial production of novel sulphated alkaloids for drug discovery. Sci Rep. 8 :7980. (2018)

Nakamura T, Kinjo C, Nakamura Y, Kato Y, Nishikawa M, Hamada M, Nakajima N, Ikushiro S, Murota K. Lymphatic metabolites of quercetin after intestinal administration of quercetin-3-glucoside and its aglycone in rats. Arch Biochem Biophys. 645:126-136. (2018)

Yasuda K, Tohyama E, Takano M, Kittaka A, Ohta M, Ikushiro S, Sakaki T. Metabolism of 2α-[2-(tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D3 by CYP24A1 and biological activity of its 24R-hydroxylated metabolite. J Steroid Biochem Mol Biol. 178:333-339. (2018)

Nishikawa M, Masuyama Y, Nunome M, Yasuda K, Sakaki T, Ikushiro S. Whole-cell-dependent biosynthesis of sulfo-conjugate using human sulfotransferase expressing budding yeast. Appl Microbiol Biotechnol. 102:723-732. (2018)

Mano H, Ikushiro S, Saito N, Kittaka A, Sakaki T. Development of a highly sensitive in vitro system to detect and discriminate between vitamin D receptor agonists and antagonists based on split-luciferase technique. J Steroid Biochem Mol Biol. 178:55-59. (2018)

Horiuchi H, Usami A, Shirai R, Harada N, Ikushiro S, Sakaki T, Nakano Y, Inui H, Yamaji R. S-Equol Activates cAMP Signaling at the Plasma Membrane of INS-1 Pancreatic β-Cells and Protects against Streptozotocin-Induced Hyperglycemia by Increasing β-Cell Function in Male Mice. J Nutr. 147:1631-1639. (2018)

受賞など

2023年度日本ビタミン学会奨励賞　(西川美宇(助教), 2023)
第1回環日本海生命科学研究会 最優秀発表賞 (中段晴太(M2), 2023)　
日本生化学会北陸支部大会第41回大会 学生ベスト発表賞(中段晴太(M2), 2023)
日本薬学会第143年会学生優秀発表賞 (中段晴太(M2), 2023)
令和4年度内外環境応答・代謝酵素研究会　学生優秀発表賞  (釘宮優希(B4), 2022)
2020年度日本フードファクター学会学術集会 YIA（Young Investigator Award） 若手優秀発表賞 (中山舞(M2), 2020）　
日本ビタミン学会第71回大会　学生優秀発表賞 (中山舞(M1), 2019）
20th International Conference on Cytochrome P450.  Best Poster Award（西川美宇（PD）, 2017）
日本ビタミン学会第69回大会　学生優秀発表賞　(阿部圭祐(M1),2017)
日本ビタミン学会第68回大会　学生優秀発表賞（高松将士(M1), 2016）
日本ビタミン学会第68回大会　学生優秀発表賞（遠山枝李(M2), 2016）
JSoFF2016 Young Investigsting Award (高平梨可(M1), 2016）
The 6th International Conference on Food Factors. Travel grant(高平梨可(M1), 2015）
平成27年度内外環境応答・代謝酵素研究会  優秀研究発表根岸賞（西川美宇(PD), 2015）
日本ビタミン学会第67回大会　学生優秀発表賞（高平梨可(M1), 2015）
第29回(平成24年度) とやま賞（鎌倉昌樹（講師), 2012）
JSoFF2010 Young Investigsting Award (増山優香(M2), 2010）